Monoclonal antibodies specific for simian virus 40 tumor antigens
نویسندگان
چکیده
منابع مشابه
Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen.
We isolated 16 new monoclonal antibodies that recognize large T antigen of simian virus 40 and mapped the epitopes to three distinct regions of the large T antigen. Also, 3 of the 16 recognized the large T antigen of the human papovavirus BKV.
متن کاملAntibodies specific for the carboxy- and amino-terminal regions of simian virus 40 large tumor antigen.
Antibodies specific for the amino- and carboxy-terminal portions of simian virus 40 large tumor (T) antigen were obtained by immunization of rabbits with synthetic peptides corresponding to these regions. The amino-terminal synthetic peptide has the sequence Ac-Met-Asp-Lys-Val-Leu-Asn-Arg-(Tyr). The tyrosine residue was introduced in order to couple the peptide to bovine serum albumin with bis-...
متن کاملSomatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40.
We have produced somatic cell hybrids between mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase IMP: pyrophosphate phosphoribosyltransferase; EC 2.4.2.8) and spleen cells derived from mice primed with either syngeneic or allogeneic cells transformed by simian virus 40. Such hybrids produced antibodies specific for simian virus 40 tumor (T) antigen. Only four of twelve inde...
متن کاملQuantitative in vitro measurement of simian virus 40 tumor-specific antigens.
Humoral antibodies, specific for the SV40 tumor-specific transplantation antigen, can be detected by an in vitro cytotoxic assay. (51)Cr-labeled SV40-transformed target cells are selectively killed in the presence of specific antibody and rabbit complement. Inhibition of cytotoxicity can be used to measure the antigen concentration and specific activity on different SV40-transformed cell lines....
متن کاملInhibition of the synthesis of simian virus 40 antigens in cells preinfected with Yaba tumor virus.
The synthesis of tumor and viral antigens after infection of an established line of cynomolgus monkey kidney cells with simian virus 40 (SV40) was compared in cells previously infected with Yaba virus and in cells not preinfected. SV40 failed to induce synthesis of tumor or viral antigens in cells preinfected with Yaba virus. The inhibitory state in preinfected cells was shown to develop sequen...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1981
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.39.3.861-869.1981